Nowadays, there are many plants in our community and environment, some these plants are used as spices, home perfumes or even decorations. But yet, we don’t know there other uses and importance from within.
This research study aims to determine the bioactivity of Madre Cacao (Gliricidia sepium) through lethality assay of brine shrimp. Determining the bioactivity of a plant can lead to the discoveries of other uses of the plant mostly in the field of medicine.
Madre Cacaos (Gliricidia sepium) are usually used in the skin as insect repellant and so the researchers proposed to have the study because of its potential in bioactivity.
Lethality assay of brine shrimp uses brine shrimps to be able to determine how bioactive a certain plant is. They are use in this field because of their characteristics in reacting through bioactive substances.
Brine shrimps are hatched for two days and after that, preparations of the test solutions are made. The researchers prepared three test-solutions: 1000-ppm; 100-ppm; 10-ppm. After getting the number of mortality on different concentrations and the data is tabulated in a table form and then plotted in a graph. The researchers use the table in finding the LC56 of experimental set-up.
Results from the LC56 table shows that the number of concentration is directly proportional to the number of mortality.
From the result, it was then concluded that the leaves of Madre Cacao (Gliricidia sepium) extract is bioactive.
This research study aims to determine the bioactivity of Madre Cacao (Gliricidia sepium) through lethality assay of brine shrimp. Determining the bioactivity of a plant can lead to the discoveries of other uses of the plant mostly in the field of medicine.
Madre Cacaos (Gliricidia sepium) are usually used in the skin as insect repellant and so the researchers proposed to have the study because of its potential in bioactivity.
Lethality assay of brine shrimp uses brine shrimps to be able to determine how bioactive a certain plant is. They are use in this field because of their characteristics in reacting through bioactive substances.
Brine shrimps are hatched for two days and after that, preparations of the test solutions are made. The researchers prepared three test-solutions: 1000-ppm; 100-ppm; 10-ppm. After getting the number of mortality on different concentrations and the data is tabulated in a table form and then plotted in a graph. The researchers use the table in finding the LC56 of experimental set-up.
Results from the LC56 table shows that the number of concentration is directly proportional to the number of mortality.
From the result, it was then concluded that the leaves of Madre Cacao (Gliricidia sepium) extract is bioactive.
Graph:
LD50 = 2.92
The graph shows that the comparison between the concentration and the mortality of different test tubes. And base on the LD50, it shows that the experiment shows bioactivity.
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